The miRNA expression levels in Dox-resistant EWS cells. A) The relative miRNA expression in Dox-resistant VH-64/ADR compared to parental VH-64 cells. The 46 miRNAs are listed in descending order. B) qRT-PCR was performed to confirm the differences in the expression of miR-125b in two different Dox-resistant cell lines. RNU6B was used as an internal control. The data represent the means of three separate experiments. The results are the means ± SD. *, P < 0.05. C) qRT-PCR was performed to confirm the differences in the expression of miR-125b in EWS tumors before and after treatment. A comparison was made between the EWS tumors before (open biopsy sample) and after (resection sample) VDC-IE or VAIA chemotherapy. RNU6B was used as an internal control. The gene expression levels were normalized to the average miR-125b expression level of the open biopsy samples. The results are given as the means (solid lines) ± SD (dashed lines). *, P < 0.05.