Figure 2

The effects of miR-125b on the Dox-induced cytotoxicity in EWS cells. An antisense (miR-125b and miR-93) or control construct was introduced into the Dox-resistant cells A), and the parental cells B) using a lentivirus. The cells were cultured with puromycin for 2 weeks to ensure a stable knockdown, and then were seeded at 2 × 10³ cells/well in 96 well plates. Twelve hours later, the cells were treated with various concentrations of doxorubicin for an additional 48 h. The cell viability was detected by the CellTiter-GloTM Luminescent Cell Viability Assay. The data represent the means of three separate experiments. The results are the means ± SD. *, P < 0.05. C) Cells were seeded at 2 × 10³ cell/well in 96 well plates 36 h after transfection with 100 nM control or has-miR-125b miRNA Precursor. The cells were treated with various concentrations of doxorubicin for an additional 48 h. The cell viability was detected by the CellTiter-GloTM Luminescent Cell Viability Assay. The data represent the means of three separate experiments. The results are the means ± SD. *, P < 0.05.