Figure 4

Induction of apoptosis by compound K in Kasumi-1 cells. A. Cell apoptosis following treatment with 20 μM compound K for indicated duration was quantified by using photometric enzyme-immunoassay based on measuring cytoplasmic histone-associated-DNA-fragmentation. Column: fold of induced apoptosis index compared to untreated control. Bar: SEM. *: p < 0.05. B &C. Western blot analysis of γH2AX, cleaved PARP and cleaved caspase-3. Kasumi-1 cells were treated with different doses of compound K as indicated for 20 hr and then subjected to Western blot analysis.