Skip to main content
Figure 1 | Cancer Cell International

Figure 1

From: Beclin1 inhibition promotes autophagy and decreases gemcitabine–induced apoptosis in Miapaca2 pancreatic cancer cells

Figure 1

Detection of Beclin1 expression in Beclin1-siRNA transfected Miapaca2 cells. A. Miapaca2 cells transfected with FAM-siRNA for 6 h exhibited green fluorescent signals under an inverted fluorescence microscope. B. Beclin1 protein expression analysis through Western blotting. Cytoplasmic protein was extracted from the cells of negative control siRNA (NC-siRNA) transfectant and Beclin-siRNA transfectant. The results are representative of at least three independent experiments. Beclin-siRNA3 was found the preferable sequence. **p < 0.01 vs NC-siRNA group. The Student’s t-test was used to analyze the data. C. Beclin1 mRNA expression analysis through real-time PCR, GAPDH was used to ensure equal loading. ** p < 0.01 vs NC-siRNA group. The Student’s t-test was used to analyze the data.

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us