Inhibition expression of Beclin1 increases LC3-II protein expression and formation of autophagosome in Miapaca2 cells. A. Beclin1 inhibition increased autophagy in Miapaca2 cancer cells. Western blot analysis of the LC3, Beclin1 expression in Miapaca2 cells transfected with Beclin1-siRNA and NC-siRNA for 48 h. β-Actin was used to ensure equal loading. B. Confocal laser scanning microscope for analysis of punctate pattern of LC3 localization in the cells transiently transfected with the GFP-LC3-vector and treated vehicle (Beclin1-siRNA) or control (NC-siRNA) for 48 h. The cells were stained with DAPI. (a:Miapaca2 cells transfected with NC-siRNA; b:Miapaca2 cells transfected with Beclin1-siRNA).