Figure 5
Down-regulation of Beclin1 decreases the cytotoxic sensitivity of Miapaca2 cells to Gemcitabine. A. After transfection of vector for 24 h then 24 h of drug incubation in Miapaca2 cells, majority cells went to death. Apoptosis was determined by flow cytometry. The apoptotic rate was significantly decreased to (0.06 ± 0.00)% compared with (29.13 ± 0.47)% of non- drug-treated cells (p < 0.01). B. Cell cycle was analyzed by flow cytometry. The percentage of Miapaca2 cells treated with Beclin1-siRNA plus drug in the G2 phase was (6.46 ± 1.91)% compared with (0.01 ± 0.00)% of NC-control cells; (75.07 ± 1.63)% compared with (71.00 ± 0.45)% in the G1 phase, and in the S phase was (18.48 ± 0.28)% compared to (29.00 ± 0.45)% accordingly (p < 0.01).