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Figure 6 | Cancer Cell International

Figure 6

From: Functional disruption of macrophage migration inhibitory factor (MIF) suppresses proliferation of human H460 lung cancer cells by caspase-dependent apoptosis

Figure 6

Flow cytometric determination of the effect of MIF siRNA on Apoptosis of H460 cells. (A); Cultured H460 cells were divided into three groups: A: cells transfected with NC siRNA, (B) and (C); cells transfected with MIF siRNA (siRNA1 or siRNA2 respectively). After a 48 h treatment, the cells were harvested for quantitation of apoptosis by determining changes in the cell surface expression of Annexin-V. (B) shows a description of the observed frequency of cells undergoing apoptosis which were found to be much higher in the MIF siRNA1 (18.09 ± 0.41%) and MIF siRNA2 (23.38 ± 2.67%) treated groups than in the negative control (NC) treated group (5.87 ± 1.05 and p < 0.05) of H460 cells respectively.

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