Figure 3

MEK inhibitors reversed MRP1 and MRP3 expression. A: HepG2 and Huh7 were treated with four different concentrations (2.5 μM, 5 μM, 10 μM and 20 μM) of U0126. After 48 hours of U0126 treatment, the cells were lysed and western blot was performed. B: HepG2 and Huh7 were treated with four different concentrations (1 μM, 2 μM, 5 μM and 10 μM) of AZD6244. After 48 hours treatment, the cells were lysed and western blot was performed. C: HepG2 and Huh7 were treated with gemcitabine (114 μg/ml) or doxorubicin (0.6 μg/ml) for 48 hours, and followed by another 24 hours of U0126 (10 μM) treatment. Then the cells were lysed and western blot was performed. D: HepG2 and Huh7 were treated with gemcitabine (114 μg/ml) or doxorubicin (0.6 μg/ml) for 48 hours, and followed by another 48 hours of AZD6244 (5 μM) treatment. Then cells were lysed and western blot was performed.