Notch1 and Foxp3 protein expression in tumor tissues. Tumor tissues in T-ALL mice were collected immunohistochemical assay. Samples were treated with rabbit polyclonal anti-Notch1 and anti-Foxp3. Both Notch1 and Foxp3 protein were detected in T-ALL mice and Foxp3 protein was detected mostly around tumor tissues. Image-pro plus was used to evaluate the expressions of Notch1 and Foxp3 through immunohistochemical staining. Protein expression was measured in IOD. Notch1 and Foxp3 protein expression in T-ALL mice were significantly higher than the control (P < 0.05).