Jurkat cell viability after DAPT treatment. Jurkat cell viability assay was performed by CCK8 method and the inhibition rate was analyzed. Jurkat cells were treated with increasing concentrations of DAPT (1, 2.5, 5, 10, 20 μM). The proliferation rate of Jurkat cell increased as the concentration of DAPT increased after 24 and 48 hours stimulation, especially the 48-hour time point. The inhibition was in a concentration-dependent manner with the greatest effect observed at 20 μM DAPT.