Figure 8
From: Notch1 signaling is involved in regulating Foxp3 expression in T-ALL
Expression of Hes1 , Notch1-Cleaved and Hes-1 protein after DAPT treatment. A: Real-Time PCR was used to assess Hes-1 gene expression. Hes1 was down regulated in Jurkat cells treated with 10 μM DAPT for 24, 48 and 72 hours, especially for 48 hours. B: At 48 hours time point, DAPT had the greatest effect on Hes1 expression when its concentrations were 10 μM. C: Notch1-Cleaved and Hes-1 protein expression was assessed by western blot. At 48-hours treatment with 10 μM DAPT, Notch1-Cleaved and Hes-1 protein expression was lower than the control group (P < 0.05).