Foxp3 gene expression after DAPT treatment. A: Foxp3 gene expression was down regulated as the concentrations of DAPT increased compared to the control. DAPT had the greatest effect on Foxp3 expression when DAPT was 10 μM. B: Jurkat cells were treated with DPAT at 10 μM for 24, 48 and 72 hours and DAPT had the greatest effect at 48-hours time point. After 72 hours, Foxp3 expression was up regulated.