EGFR inhibition by AG1478 did not interfere with cell proliferation. (A) EGFR inhibition by AG1478 after 1 hour or 48 hours of treatment. (B) Determination of the cell numbers after AG1478 inhibition. A549 and HK2 cells were cultured in medium containing 10% FCS, 2.5% FCS, 2.5% FCS plus EGF (100 ng), 2.5% FCS plus AG1478 and EGF or 2.5% FCS plus AG1478. The cells were counted after 48 hours. Five thousand cells from each group were analyzed in all experiments. All results are representative of three or more independent experiments. *p ≤ 0.05. Bars = standard deviation.