Analysis of mRNA and protein related to EMT. (A) Vimentin, cytokeratin-18 and E-cadherin expression in A549 and HK2 cells by Western blotting. The groups analyzed were: C10%FCS, C2.5%FCS, 2.5%FCS + EGF and 2.5%FCS + AG1478. Tubulin was used as loading control. (B) Immunofluorescence was performed to C10%FCS, C2.5%FCS and C2.5%FCS + EGF groups using an antibody against E-cadherin (green) and the nuclei were stained with propidium iodide (red). (C) RT-PCR quantification of mRNA levels of vimentin, cytokeratin-18, E-cadherin and N-cadherin in A549 and HK2 cells. The groups were the same described in A. Bars = standard deviation.