Effect of paclitaxel and SB-T-1216 on the activity of caspase-2 in SK-BR-3 and MCF-7 cells. After 0, 12, 24, 36, 48 and 60 h of incubation with paclitaxel or SB-T-1216 (100 nM for SK-BR-3 cells and 300 nM for MCF-7 cells), the activity of caspase-2 was measured by flow cytometry employing CaspGLOWTM Active Caspase Staining Kit (see “Materials and Methods“). Mean fluorescence at 0 h was 23.9 for SK-BR-3 cells and 28.5 for MCF-7 cells. Each column represents the mean of 2 experimental values ± SEM. *P < 0.05, **P < 0.01 when comparing the effect with that at 0 h.