Figure 4

5’-Aza-2-deoxycytidine and HDAC inhibitor regulate biological behaviors of EC cells . A. Western blots showing DICER1, E-cadherin and vimentin expression levels in Ishikawa and AN3CA cells treated with 10 μM 5'-aza-CdR and 10 μM TSA. B. The proliferation of AN3CA cells treated as indicated. C. The cell cycle prolifes of AN3CA cells treated as indicated. D. The colony formation of AN3CA cells treated as indicated. E. Transwell invasion assay of AN3CA and Ishikawa cells treated as indicated. Representative images of AN3CA and Ishikawa cells were shown (magnification × 200). Statistical analysis of the number of invaded Ishikawa and AN3CA cells. F. in vitro wound healing assay of AN3CA cells. Photographs were taken at 0, 6 and 24 h after the wound was made.