Table 1 Bisulfite specific PCR sequencing of miRNAs
From: CpG island hypermethylation-associated silencing of microRNAs promotes human endometrial cancer
GENE | Cell type | Bisulfite specific PCR sequencing | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CpG-1 | CpG-2 | CpG-3 | CpG-4 | CpG-5 | CpG-6 | CpG-7 | |||||||||
gene locus | probability | gene locus | probability | gene locus | probability | gene locus | probability | gene locus | probability | gene locus | probability | gene locus | probability | ||
miR-130a | AN3CA | 26 | 100% | 120 | 100% | 227 | 100% | 276 | 0 | / | / | / | / | / | / |
Ishikawa | 80% | 100% | 80% | 0 | / | / | / | ||||||||
miR-130b | AN3CA | 33 | 0 | 46 | 40% | 119 | 80% | 151 | 100% | 183 | 100% | / | / | / | / |
Ishikawa | 80% | 100% | 80% | 80% | 80% | / | / | ||||||||
miR-625 | AN3CA | 24 | 100% | 17 | 100% | 53 | 100% | 68 | 100% | 90 | 100% | / | / | / | / |
Ishikawa | 100% | 80% | 100% | 60% | 100% | / | / | ||||||||
miR-200b | AN3CA | 26 | 29% | 55 | 100% | 77 | 86% | 81 | 86% | 88 | 86% | 99 | 86% | 107 | 86% |
Ishikawa | 40% | 100% | 100% | 80% | 80% | 80% | 60% | ||||||||
miR-222 | AN3CA | 122 | 100% | / | / | / | / | / | / | / | / | / | / | / | / |
Ishikawa | 100% | / | / | / | / | / | / | ||||||||