A-C Western blot analysis of p35 and p25 expression in cultured hippocampal neurons at different time after irradiation. Representative Western blot showing the expression of p35 and p25 in cultured hippocampal neurons exposed to a single dose of 30 Gy irradiation, and tested at 3.5 h, 4 h, 5 h, and 6 h after irradiation (A). Expression of p35 and p25 was tested at3.5 h after sham-irradiation as control. Quantitative Western blot analysis of p35 (B) and p25 (C) expression in cultured hippocampal neurons with sham-irradiation, and with 30 Gy irradiation at 3.5, 4 h, 5 h, and 6 h after irradiation. β-actin was a loading control. n=4. *p<0.05 vs. control.