Up-regulation of miR-146a expression can inhibit NF-κB activity in BGC-823, SGC-7901 and MGC-803 cells. The real-time PCR revealed that miR-146a mimic significantly increased the expression of miR-146a (A). Western blot showed that transfected miR-146a mimic decreased phosphorylation of IκB and nuclear p65 level (B). The luciferase reporter assay revealed that transfected miR-146a mimc significantly repressed NF-κB transcriptional activity (C). *P < 0.05, indicate significant differences from the respective control groups.