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Figure 4 | Cancer Cell International

Figure 4

From: Celastrol induces apoptosis of gastric cancer cells by miR-146a inhibition of NF-κB activity

Figure 4

Down-expression of miR-146a can reverse the effect of celastrol on NF-κB activity and apoptosis. The real-time PCR revealed that miR-146a inhibitor can significantly decrease the expression of miR-146a in BGC-823, SGC-7901 and MGC-803 cells (A). After transfected with 100 nmol/L of miR-146a inhibitor, the cells were treated with 2 μM celastrol for 6 h. miR-146a inhibitor significantly increased NF-κB transcriptional activity in BGC-823, SGC-7901 and MGC-803 cells after celastrol treatment (B). Flow-cytometric analysis showed that miR-146a inhibitor significantly decreased the apoptosis of BGC-823, SGC-7901 and MGC-803 cells induced with celastrol (C). After transfected with 100 nmol/L of miR-146a inhibitor, the cells were treated with 2 μM celastrol for 72 h. MTT revealed that miR-146a inhibitor significantly decreased the effect of celastrol on inhibition of BGC-823, SGC-7901 and MGC-803 cells growth (D). *P < 0.05, indicate significant differences from the respective control groups.

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