Table 1 RT-qPCR primers used in the present study
From: MiR-145 inhibits oral squamous cell carcinoma (OSCC) cell growth by targeting c-Myc and Cdk6
Gene | Primer sequences (5’-3’) |
|---|---|
MiR-145 | RT primer: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACagggatt |
| Â | Forward primer: CAGTGCGTGTCGTGGAGT |
| Â | Reverse primer: AGGTCCAGTTTTCCCAGG |
U6 | RT primer: CGCTTCACGAATTTGCGTGTCAT |
| Â | Forward primer: GCTTCGGCAGCACATATACTAAAAT |
| Â | Reverse primer: CGCTTCACGAATTTGCGTGTCAT |
c-Myc | Forward primer: GCTGCTTAGACGCTGGATTT |
| Â | Reverse primer: CACCGAGTCGTAGTCGAGGT |
Cyclin D1 | Forward primer: AGACCTTCGTTGCCCTCTGT |
| Â | Reverse primer: AGTTGTTGGGGCTCCTCAG |
Cdk6 | Forward primer: TGGAGACCTTCGAGCACC |
| Â | Reverse primer: CACTCCAGGCTCTGGAACTT |
β-actin | Forward primer: GCACAGAGCCTCGCCTT |
| Â | Reverse primer: GTTGTCGACGACGAGCG |