Effects of BAD overexpression on NSCLC cells cycle. Approximately 5 × 105 to 1 × 106 cells were harvested and stained using propidium iodide. DNA content was determined by flow cytometry. Aggregated cells were gated out and cell cycle profiles were analyzed to quantitate cell cycle distribution. No significant effects on the cell cycle distribution were observed in all cell lines (all p > 0.05).