Effects of BAD overexpression on NSCLC cell apoptosis. Cells were harvested and stained with V-APC/7AAD. Apoptotic rates were caucluated by flow cytometry (A). The Annexin V-positive and 7AAD-negative cells (Q4) were regarded as apoptotic (B). For the xenograft mice, paraffin-embedded sections of H1299 and SPC-A1 tumor were subjected to TUNEL assay. The amount of TUNEL fluorescence in five random view was quantified (C, all p < 0.05). Representative photomicrographs of TUNEL staining were shown (D). Apoptotic neuclei showed red staining (top), and nuclei stained with DAPI in blue (bottom). The merged images of nuclei in pink (arrows) indicate apoptotic cells. Original magnification, × 200, Bar: 50 μm.