Effect of apigenin on T24 cell invasion and migration. Cells treated with 0–20 μM apigenin were induced to move or invade through uncoated or Matrigel-coated transwell membranes. After 24 hours, cells were fixed, stained, and counted. A, a representative image; B, shows the quantitation of one experiment done in triplicate. * P < 0.05 versus untreated control, ** P < 0.01 versus untreated control.