Effects of TSA on apoptosis in DLBCL cells. (A) Annexin V/PE-7AAD dual staining and flow cytometric analysis of early and late apoptosis of LY1, LY8 and DoHH2 cells treated with TSA (concentrations as indicated) for 24 h. One representative experiment shown for each cell line. The lower right quadrant shows percentage of cells in early apoptosis, the upper right quadrant indicates percentage of cells in late apoptosis, and the lower left quadrant shows percentage of vital cells. (B) Percentages of early apoptosis cells are displayed as mean ± SD of three independent experiments performed in triplicate. * P < 0.05 compared with vehicle treatment.