Effect of relaxin-2 knockdown on osteosarcoma MG-63 cell proliferation and apoptosis. A, Cell viability was determined by MTT. The growth rate of MG-63 cells after relaxin-2 knockdown with siRNA for 48 hs was significantly decreased in MTT assay. B, Apoptotic cells was determined by TUNEL analysis in MG-63 cells following relaxin-2 knockdown with siRNA for 48 hs. The apoptotic cells in relaxin-2 siRNA transfectants were significantly higher compared to MG-63-non-transfection or MG-63-siRNA control cells respectively. Columns, mean values from triplicate experiments; Each bar represents mean ± SE (n = 3); *, P < 0.05 versus MG-63-non-transfection or MG-63-siRNA control.