Results of real-time PCR. A. Amplified curve analysis of actin. B. Melting curve analysis of actin. C. Amplified curve analysis of NOTCH1. D. Melting curve analysis of NOTCH1. E. Compare of 2-△△C T value of ALDH1A1 and NOTCH1 expression in each case. F. Compare of mean 2-△△C T value of NOTCH1 expression regarding to ALDH1A1 expression (using a cut-off value based on the median ALDH1A1 2-△△C T value).