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Table 4 Measurement of ALDH1A1 and NOTCH1 mRNA with real-time PCR a

From: Expression of ALDH1 in breast invasive ductal carcinoma: an independent predictor of early tumor relapse

Target ALDH1A1 NOTCH1 β-actin
n 52 52 52
Avg. CT 22.55 ± 1.21 (19.25~25.07) 28.12 ± 1.65 (24.62~31.76) 18.19 ± 2.16 (14.63~22.80)
CT −1.03 ± 0.21 (− 4.33~1.49) −0.50 ± 0.65 (− 4.01~3.14) −1.11 ± 0.16 (− 4.67~3.50)
CT 0.08 ± 0.01 (− 2.56~4.37) 0.61 ± 0.03 (− 2.85~4.49)  
2-CT 1.75 ± 0.70 (0.05~5.88) 1.29 ± 0.58 (0.04~7.19)  
  1. a Total RNA was purchased from Clontech, and cDNA synthesized from 1 mg total RNA using reverse transcriptase. Aliquots of cDNA were used as the template for real-time PCR reactions containing either primers and probe for ALDH1A1 or NOTCH1 and probe for β-actin. Each reaction included cDNA derived from 10 ng total RNA, and six replicates per reaction were performed.