Figure 1From: Genistein promotes cell death of ethanol-stressed HeLa cells through the continuation of apoptosis or secondary necrosisLow concentration ethanol stressed HeLa cells displayed apoptotic features but could recover after stress removal. (a). Live cell imaging of HeLa cells before stress treatment (control), 5.5% ethanol stressed for 6 (E6 hrs) and 8 h (E8 hrs), and after stress removal for 24 h (R24 hrs). Mitochondria (red, MitoTracker Red Staining) and Nuclei (blue, Hoechst 33342 staining) were visualized by fluorescence (Scale bar: 20 μm). (b). Western blot was used to detect the cleavage of PARP and caspase enzymes after stress treatment (E6 hrs & E8 hrs) and 24 h recovery (E6 hrs + R24 hrs & E8 hrs + R24 hrs). (c). Immunostaining of cleaved caspase-3 of stressed (E) and recovered (E + R) HeLa cells. After incubated with anti-cleaved caspase 3 antibody, the cells were further stained with Alexa Fluor® 488-conjugated secondary antibody. Nuclei were stained with Hoechst 33342 and the cells were visualized with fluorescence microscopy (Scale bar: 100 μm).Back to article page