Genes involved in the recovery from stress treatment were influenced by genistein. (a). Dose-dependent responsive curves of Actinomycin D and Cycloheximide on unstressed cells (ACD Alone and CHX Alone) and stressed cells (E8 hrs + ACD and E8 hrs + CHX) of HeLa for 48 h treatment by MTT assay. Two-way ANOVA was used to test the significance of ethanol stress as the source of variance. IC50 of each group is summarized in table. (b). Schematic schedule of RNA sample collection for each treatment group, including genistein treatment alone, stressed and replaced with fresh medium for recovery, and stressed and replaced with genistein-containing medium. (c). Time-dependent gene expression profiles of ATF3, CREB1, XIA P, MDM2, TP53, NOXA, PUMA, MCL1, BAX, BCL2, in different groups of HeLa cell. Control stands for the untreated cells; E8 hrs represents ethanol-stressed cells for 8 hrs; Black line: ethanol-stressed and stress replaced with fresh medium; Orange line: ethanol-stressed and stress replaced with medium containing 15 μg/ml genistein. Blue line: unstressed cells treated with 15 μg/ml genistein. Each gene expression level is normalized to that of GAPDH. (Data point above is expressed as Mean ± SEM, n = 3; asterisk indicates statistical comparison of each time point between Wash + Fresh Medium and Wash + Genistein group by tow-way ANOVA, * indicates: p < 0.05; ** indicates: p < 0.01).