Figure 5From: Genistein promotes cell death of ethanol-stressed HeLa cells through the continuation of apoptosis or secondary necrosisCaspase activity inhibition did not rescue the effects of genistein’s effect on recovering HeLa cells. (a). HeLa cells were stressed or unstressed with 5.5% ethanol for 8 h (E8 hrs), in the absence (−) or the presence (+) of 25, 50, and 100 μM of general caspase inhibitor Z-VAD-fmk, and the PARP cleavage was examined by Western blot. (b). MTT assay about genistein’s effects on stressed HeLa cell, in the absence (−) or the presence (+) of general caspase inhibitor Z-VAD-fmk (100 μM) for 24 h. E 8 hrs stands for 8 h ethanol stress; Washing stands for the removal of ethanol-containing medium (Data expressed as Mean ± SEM, n = 3; ns indicates: no significance; ** indicates: p < 0.01).Back to article page