Table 1 Primer sequences for target genes
Gene symbol | Forward Primer (5’ to 3’) | Product size |
|---|---|---|
Reverse Primer (5’ to 3’c | ||
BCL2 | ATGTCCAGCCAGCTGCACCTGAC | 319Â bp |
GCAGAGTCTTCAGAGACAGCCAGG | ||
MDM2 | CCCTGGTTAGACCAAAGCCAT | 190Â bp |
GGCACGCCAAACAAATCTCC | ||
GAPDH | CATGAGAAGTATGACAACAGCCT | 113Â bp |
AGTCCTTCCACGATACCAAAGT | ||
XIAP | GGGTTCAGTTTCAAGGACATTAAG | 182Â bp |
CGCCTTAGCTGCTCTTCAGTAC | ||
ATF3 | AACCTGACGCCCTTTGTCAAG | 138Â bp |
TACCTCGGCTTTTGTGATGGA | ||
BAX | GATGCGTCCACCAAGAAGCT | 170Â bp |
CGGCCCCAGTTGAAGTTG | ||
MCL1 | TAAGGACAAAACGGGACTGG | 137Â bp |
ACCAGCTCCTACTCCAGCAA | ||
TP53 | GAGGTTGGCTCTGACTGTACC | 133Â bp |
TCCGTCCCAGTAGATTACCAC | ||
CREB1 | TTAACCATGACCAATGCAGCA | 140Â bp |
TGGTATGTTTGTACGTCTCCAGA | ||
PUMA | GACCTCAACGCACAGTACGAG | 98Â bp |
AGGAGTCCCATGATGAGATTGT | ||
NOXA | ATGCCTGGGAAGAAGGCGC | 164Â bp |
CAGGTTCCTGAGCAGAAGAGT |