Figure 3

QNT11 induces apoptosis in Hep3B cells. Induction of apoptosis in Hep3B cells treated with QNT11 for 24 h was evaluated by TUNEL assay. Representative images were taken with nuclear stain DAPI, (A) and apoptosis stain TUNEL, (B) × 200. (C) Data are expressed as overall means ± SD from three independent experiments. Statistical significance was determined using the Student’s t-test (* P < 0.05, ** P < 0.01 vs control). (D) Hep3B cells treated with 0, 0.803 ( IC₃₀ group), 2.210 (IC₅₀ group) and 3.616 μM (IC₇₀ group) QNT11 respectively for 24 h. DNA from 1 × 10⁶ cells was electrophoresed through 1% agarose gels and stained with 0.5 μg/ml ethidium bromide.