Figure 2

Down-regulation of miR-214 inhibits the proliferation, migration and invasion of BGC-823 gastric cancer cell lines. (A) Cell viability was measured at 24 hr post-transfection by MTT assay. Results showed that MTT value of cells transfected with anti-miR-214 was significantly lower than that of cells transfected with control anti-miRNA after 48 hr post-transfection. (B) Cell population doubling time (PDT) was determined as described in materials and methods with cells at 24 hr post-transfection. Results showed that the PDT of cells transfected with anti-miR-214 was significantly longer than that of cells transfected with control anti-miRNA. (C, D) Cell cycle analysis was performed at 48 hr post-transfection by staining DNA with propidium iodide prior to flow cytometry. Results showed that proliferative index (PI) of cells transfected with anti-miR-214 was significantly lower than that of cells transfected with control anti-miRNA. (E, F) Clonogenic assay was performed as described in materials and methods with cells at 24 hr post-transfection. Results showed that the colony forming efficiency (CFE) of cells transfected with anti-miR-214 was significantly lower than that of cells transfected with control anti-miRNA. (G, H) Gastric cancer cells transfected with anti-miR-214 and control anti-miRNA were seeded into the upper part of a transwell chamber with or without matrigel, cells migrating across the membrane were counted in all fields. Results showed that number migrating across the membrane with or without matrigel of cells transfected with anti-miR-214 was significantly less than that of cells transfected with control anti-miRNA. Data represent mean ± SEM from three independent experiments; *P < 0.05 by t test, **P < 0.01 by t test.