Figure 4

Down-regulation of PTEN attenuates the effects of anti-miR-214 on BGC-823 gastric cancer cell proliferation, migration and invasion. (A, B) The expression level of PTEN protein was detected by Western-blot at 48 hr post-transfection and normalized to that of β-actin. Results showed that the expression level of PTEN protein was significantly lower in cells co-transfected with anti-miR-214 and siRNA-PTEN as compared to the cells transfected with anti-miR-214 and control siRNA. Cell vability was detected by MTT assay (C), population doubling time determination (D), proliferative index (E), clonogenic assay (F), migration and invasion assay (G, H) as described in materials and methods. Results showed that the proliferative capacity of cells co-transfected with anti-miR-214 and siRNA-PTEN was significantly higher than that of cells co-transfected with anti-miR-214 and control siRNA. Data represent mean ± SEM from three independent experiments; *P < 0.05 by t test, **P < 0.01 by t test.