Figure 12
From: Localization of BRCA1 protein in breast cancer tissue and cell lines with mutations
BRCA1 protein levels in MCF7 and HCC1937 cells. Extracts of HCC1937 and MCF7 cells were normalized for protein content, separated by 5% SDS PAGE, and immunoblotted for BRCA1 with mAb MS110. HCC1937 extracts revealed low levels of a 210 kDa BRCA1 species, when we compared 100 μg of total protein in the HCC1937 extracts (5382insC product) with 50 μg, 100 μg, and 150 μg of control MCF7 extracts (wild-type BRCA1).