DNA sequencing chromatogram illustrates the mutation, 185delAG, resulting from the deletion of two consecutive nucleotides AG at position 185 (∇) in BRCA1 gene in sample no. 13 (lower panel). DNA sequences from sample no. 12 (wild type) are shown in top panel. Arrows indicate the position of AG deletion. In both, DNA from FFPE tissues were amplified and nucleotide sequences were determined using the primers flanking the mutation loci.