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Figure 9 | Cancer Cell International

Figure 9

From: Localization of BRCA1 protein in breast cancer tissue and cell lines with mutations

Figure 9

Immunofluorescence staining of BRCA1 protein localization in poorly differentiated adenocarcinoma (PDA) from patient no. 22 and an asynchronous culture of MCF7 cells. A. The tumor is stained with rabbit polyclonal K-18 antibody and FITC-labelled goat anti-rabbit serum. Many tumor cell nuclei show both 1–5 punctate bodies (green) and large round central structures (green) and the interstitial cell nuclei are very weakly stained. B. Asynchronous culture of MCF7 cells stained with rabbit polyclonal K-18 antibody and FITC-labelled goat anti-rabbit. BRCA1 staining consisting of fluorescent nuclei, intra-nuclear punctate bodies (green) and cytoplasmic staining. (×300).

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