Figure 1From: Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell lineEGF-induced EMT in MDA-MB-468 breast cancer cells. MDA-MB-468 breast cancer cells were serum starved and treated with EGF (50 ng/mL) as indicated to induce EMT. A) Twist and B) vimentin mRNA expression were assessed using real time RT-PCR at 3, 6 and 12 h following EGF treatment. Bar graphs show mean ± S.D. for nine individual wells from three independent experiments. The effect of EGF at each time point was assessed using two-way ANOVA with Bonferroni’s multiple comparisons post-tests. * P < 0.05.Back to article page