Figure 4From: Functional features of cancer stem cells in melanoma cell linesLimiting dilution analysis and clonogenic assay. A: Limiting dilution analysis. Percentage of positive wells (positive well = 1 cell colony/well) at different cell concentrations of CD133+ and CD133- D10 cells for calculating Poisson’s distribution (Figure 4B). B: Clonogenic capacity of D10 cells. Results of Poisson’s distribution with the frequency of proliferating cells in CD133+ (black column) and CD133- (white column) D10 cells, results expressed as mean percentages ± SD; (*) = p ≤ 0.001.Back to article page