Functional features of cancer stem cells in melanoma cell lines

Table 1 **Expression of MAA* in melanoma cell lines**

Cell line	Melanoma differentiation antigens			Cancer/testis antigens
	gp100	tyrosinase	MART-1	MAGE-A3	NY-ESO
MZ2	-	-	-	+ +	-
D10	+	+	+ +	+ +	-
Me39	-	-	+ +	+ +	-
WM115	-	-	+	+ +	-
RE	-	-	-	+ +	-
Me59	-	-	-	+/-	-
Me67	-	-	-	+ +	-
Na8	-	-	-	-	-
HBL	+	+	+ +	+ +	-

*MAA = melanoma-associated antigens; Expression levels were assessed using conventional PCR and evaluated semiquantitatively by densitometry. gp100 = melanosomal matrix protein gp100; tyrosinase = key enzyme in melanin biosynthesis; MART-1 = Melan-A/MART-1 = melanoma antigen recognized by T cells; MAGE-A3 = melanoma antigen-A3 family, NY-ESO = cancer/testis antigen (see text); (+) = moderate expression; (+ +) = strong expression; (-) = no expression detectable.

ISSN: 1475-2867