Analysis of HeLa cell viability after 24 hour ESE-16 exposure. Apoptosis was determined by flow cytometric quantification of phosphatidylserine-flip. (3i) Dots plots of HeLa cells after 24 hour exposure to growth medium (3ia), DMSO vehicle control (3ib), actinomycin D positive apoptosis control (3ic) and ESE-15 (3id). A statistically significant decrease of viable cells was seen in actinomycin D- and ESE-16-exposed cells with a concurrent increase of apoptotic cells. There were no statistically significant differences observed between cells propagated in growth medium only and DMSO vehicle controls in any of the cell cycle categories. No significant difference was calculated in the amount of necrotic cells in all samples. (3ii) Graphical representation of the dot plot data (* P < 0.05, standard deviation represented by T-bars).