Flow cytometric evaluation of cyclin B1 up-regulation in HeLa cells. (4i) Dot plots of ESE-16 exposed cells (b and d) were compared to the DMSO vehicle control (a and c). FL3 log fluoresce intensity was plotted against cell size determined by forward scatter (a and b) and cell complexity as established with side scatter (c and d). An increase in expression of cyclin B1 in the ESE-16-treated cells was observed in the smaller (b) and more complex (d) cells. (4ii) Intensity histogram of cyclin B1 expression within the DMSO vehicle control (black) and ESE-16-exposed cells (blue) demonstrating an increase in intensity and a right shift in the drug-exposed cells.