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Figure 13 | Cancer Cell International

Figure 13

From: Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells: Does the response involve senescence? The possible role of vimentin

Figure 13

a-i Organization of vimentin in A549 cells after treatment with etoposide - fluorescence microscopic examination (vimentin indirect labeling, secondary antibody was goat anti-mouse IgG-TRITC). a Control cells, b, c 0.75 μM etoposide, d-f 1.5 μM etoposide, g-i 3 μM etoposide. Apart from regularly organized bundles of filaments in the control cells, abundant, but more heterogeneously organized structures appeared as a result of the treatment. Thick arrows point at dense, thick vimentin bundles in the form of septum-like aggregates between sister nuclei, especially common in multinucleated cells, or vimentin threads in the nuclear invaginations; double thick arrows - putative budding-like structures in close proximity of the parental cells; thin arrows - long vimentin tails; arrowheads - diffuse vimentin staining around nuclei with features of karyorrhexis or karyolysis. Nuclei were counterstained with DAPI. Results are indicative of five independent experiments. Bar 50 μm.

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Cancer Cell International

ISSN: 1475-2867

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