TLR9 signaling enhanced the expression of HuR in human lung cancer cells. (A) 95D cells were treated with indicated dose of CpG ODNs. 72 hrs later, the expression level of HuR mRNA was detected by Realtime PCR assay. (B) The expression of HuR protein also was detected by Western Blot and calculated. (C). 95D cells were treated with 10 μg/ml CpG ODNs. Then, the expression of miR-7 and HuR were analyzed at indicated time point. (D) Human lung cancer cell line BE1, NCI-H727 and SPCA/I cells were treated with 10 μg/ml CpG-ODNs respectively for 72 hrs. Then the expression level of HuR mRNA was detected by Realtime-PCR assay. One representative data of three independent experiments was shown.