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Figure 2 | Cancer Cell International

Figure 2

From: TLR9 signaling repressed tumor suppressor miR-7 expression through up-regulation of HuR in human lung cancer cells

Figure 2

Overexpression of HuR reduced the expression of miR-7 in human lung cancer cells. 5 × 104 95D cells were transiently transfected with plasmid p-HuR (100 μg) or p-Cont (100 μg) and cultured in 24-well plate. (A) 72 hrs later, the expression of HuR was analyzed by Western Blot and calculated. (B) The relative expression of miR-7 was determined by Realtime PCR assay. (C) 95D cells were transiently transfected with plasmid p-HuR (100 μg) or p-Cont (100 μg). The relative expression of miR-7 was further determined by Real-time PCR assay at indicated time point. (D) Human lung cancer cell line BE1, NCI-H727 and SPCA/I cells were also transiently transfected with 100 μg plasmid p-HuR or p-Cont and cultured in 24-well plate respectively. 72 hrs later, the relative expression of miR-7 also was determined by Real-time PCR assay. One representative data of three independent experiments was shown.

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