Figure 3

Down-regulation of HuR reversed the expression of miR-7 in CpG ODNs treated human lung cancer cells. 95D cells were transiently transfected with HuR RNAi (10 nmol) or control RNAi (10 nmol) respectively and then treated with CpG ODNs (10 μg/ml). 72 hrs later, the expression of HuR was determined by western blot and calculated (A). (B) The relative expression level of miR-7 was detected by Real-time PCR assay. One representative data of three independent experiments was shown.