Figure 4

Down-regulation of HuR reduced TLR9 signaling enhanced growth and metastatic potential of human lung cancer cells. 95D cells transiently transfected with HuR RNAi (10 nmol) or control RNAi (10 nmol) were cultured in the presence of 10 μg/ml CpG ODNs. 72 hrs later, the proliferation of cells also was determined by MTT assay (A) and BrdU incorporation assay (B). (C) The migration of 95D cells in each group was performed by transwell assay. (D) The invasion ability of 95D cells in each group also was determined by invasion assay. One representative data of three independent experiments was shown.