p-Akt and p-NFκB levels of C
-ceramide-treated H1299 lung cancer cells. Cells treated with different concentrations (0 to 50 μM) of C2-ceramide for 24 h. After treatment, the protein lysates were resolved by SDS-PAGE, transferred onto nitrocellulose membranes and probed with specific antibodies and detected signals using an enhanced chemiluminescence kit. (a) The changes of Akt and NFκB phosphorylation. (b) The changes of protein level of survivin, cyclin A2 and Bax. β-actin as an internal control.