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Figure 2 | Cancer Cell International

Figure 2

From: Sensitization of U937 leukemia cells to doxorubicin by the MG132 proteasome inhibitor induces an increase in apoptosis by suppressing NF-kappa B and mitochondrial membrane potential loss

Figure 2

Apoptosis and caspase activity in U937 cells treated with Doxorubicin (DOX), MG132, or MG132 + DOX. U937 cells were incubated alone or in combination with MG132 (1 μM), DOX 1 μM, or MG132 + DOX for 24 h at 37°C in a humid atmosphere containing 5% CO2 in RPMI-S culture medium. After incubation, the cells were washed and apoptosis was assessed by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC) (a). For each sample, at least 20,000 events were acquired in a FACSAria-I cell sorter and the data were analyzed with FACSDiva software. The activity of caspase-3 (b), -8 (c), and −9 (d) was evaluated utilizing a caspase colorimetric staining kit. The results represent the mean ± the Standard deviation (SD) of three independent experiments performed in triplicate. Statistical analysis was performed by means of the Mann–Whitney U test. ●p <0.05 MG132 + DOX vs MG132, DOX, or the Untreated control group (UCG); *p <0.05 all groups vs the UCG.

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Cancer Cell International

ISSN: 1475-2867

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